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The ORCA-R2 (C10600-10B) offers high-quality imaging over a wide spectral range, with especially high sensitivity in the visible and near-infrared regions. Its CCD sensor is encased in a maintenance-free, hermetic vacuum-sealed chamber which ensures efficient camera cooling by either air or water. Thanks to this cooling structure and an advanced external circuit design, the ORCA-10B also provides rapid readout with minimal noise. In normal scan mode, readout is 8.5 frames per second at full spatial resolution. In fast scan mode, frame rates range from 16.2 to 115.1 frames per second at varying levels of resolution. |
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- U2 with mApple H2B and mEmerald Csrc
(39.3 MB) 
- The term Src, an abbreviated form of the word sarcoma, denotes a family of tyrosine kinases that are proto-oncogenic. Generally animal cells contain at least one of the Src families of tyrosine kinases, the enzymes that are able to transfer a phosphate group from ATP to a tyrosine residue. The phosphorylation of proteins by tyrosine kinases is crucial for signal transduction in multicellular animals. V-Src is the mutated version of cellular Src (C-Src) that has lost its characteristic ability to inhibit enzyme activity. C-Src was targeted in the human osteosarcoma epithelial cells featured in the digital video sequences presented in this section with mEmerald, while H2B was fluorescently labeled with mApple.
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- Fox Lung (FoLu) mEmerald H2B and mCherry Mito
(35.7 MB)
- Mitochondria typically exhibit an oblong form and range in size between 1 and 10 micrometers in length. The organelles exhibit significant flexibility, however, and when live cells are examined it quickly becomes clear that mitochondria are almost continuously shifting shape and moving throughout the cytosol. Mitochondrial movement appears to be associated with the cellular network of microtubules. The dynamic activity of mitochondria was visualized in the digital video sequences presented in this section with mCherry fused to a mitochondrial targeting signal. The cell nucleus was targeted with a mEmerald-H2B fusion.
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- Fox Lung mEmerald Lifeact and mApple alpha-Actinin
(21.6 MB)
- A directed evolution approach was used to produce mApple and several other red fluorescent protein variants belonging to the "mFruit" series. Compared to mStrawberry, mApple is significantly more photostable, in addition to exhibiting excellent brightness and rapid maturation. The peak excitation and emission wavelengths of mApple are 568 and 592, respectively. The cytoskeletal network was targeted in the fox lung cells featured in the digital video sequences in this section with mApple fused to alpha-actinin. In addition, the cytoskeleton was labeled with a chimera of mEmerald and Lifeact, resulting in some visible overlap of the fluorescent markers.
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